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The young leaves of Camptotheca acuminata as explants were cultured in vitro. The well-healed callus tissues that had been stable after subculture were cultured under light conditions. After some time, red material appeared on the surface of the callus. A large number of documents indicate that this red material is anthocyanins. With the extension of culture time, the red color appearing on the surface of the healing tissue of C. acuminata gradually darkens. Anthocyanins are mainly composed of anthocyanins and sugars in plant cells, mainly glucosides.
The artificial climate incubator was set under light conditions. The soluble protein content in the callus of C. acuminata showed an upward trend in the first 3 weeks and reached the maximum value in the 3rd week, while the protein content decreased rapidly in the 3rd week of culture. In the pre-culture, the healing tissue was in a good state of growth and the protein synthesis and metabolism were vigorous. At the later stage of culture, with the browning and apoptosis of the healing tissue, the growth activity of Camptotheca acuminata decreased, and the soluble protein content decreased. It is generally believed that some regulatory enzymes are intracellular soluble proteins, and the synthesis and accumulation of camptothecin in Camptotheca callus is affected by these proteins.
The effect of light treatment on the physiological parameters such as soluble protein, peroxidase activity, etc. in the cell of Camptotheca acuminata was studied in the artificial climatic incubator experiment, and the effect of different treatment of light conditions on the lye was also analyzed. In addition, the unusual appearance of the different light qualities for culture and some physiological studies is not currently lacking. Therefore, in the future, the research on the effect of light quality on the synthesis and accumulation of camptothecin in Camptotheca acuminata is a research direction.
The Effect of Photoreaction on Anthocyanin in Artificial Climate Incubator
Anthocyanins are flavonoids that are widely found in plants and have many important physiological functions. The use of an artificial climatic incubator to control the light shows that anthocyanins have a clear response to light. However, there was no significant correlation between the synthetic bases for production of anthocyanins. The young leaves of Camptotheca acuminata as explants explanted in vitro will stably grow and grow well after healing. Under light conditions, red material will be found on the callus surface after some time. A large number of documents indicate that the red material is anthocyanins.
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