Method for determination of leaf area of ​​Leymus chinensis

Method for determination of leaf area of ​​Leymus chinensis

Leymus chinensis is a rhizome grass type, which is often a community-building species. Its special biological characteristics and strong resistance have always been the focus of research. Leaf area index is an important biological indicator of the physiological ecology and the cultivation and breeding of Leymus chinensis. At present, the commonly used methods for measuring the leaf area include grid method, leaf area meter method, digital image processing method, and copy weighing method. Pros and cons. When a large number of samples are taken in the field, the determination of the leaf area of ​​L. chinensis is very arduous. How to quickly and accurately determine the leaf area of ​​L. chinensis is the most direct way to increase the efficiency of L. chinensis.

The determination of leaf area is very necessary when studying plant photosynthesis. The leaf is the most important place for the existence of chlorophyll, and the photosynthesis of plants cannot be separated from chlorophyll. Therefore, measuring the area of ​​the leaves will allow us to have a deeper understanding of the plant's photosynthesis and at the same time allow us to understand the plant's yield more precisely. Therefore, leaf area measurement is essential in the physiological study of plants. Leaf area measurement has specific instruments, such as a living leaf area meter, that can determine the area of ​​any leaf other than coniferous plants, and there is no requirement for the size of the leaf area, but it has a drawback that it can cause damage to plants because of living organisms. The leaf area meter needs you to take off the leaf; the advantage of the portable leaf area meter is that it is portable, it can be carried around, and it can be measured without taking off the leaf, but it also has its disadvantage that it has requirements on the size of the leaf and cannot Determine whether the blade is too big or too small.

Long-width method for determination of leaf area: For the determination of leaf area of ​​Leymus chinensis leaves, the length-width method was used to calculate the area of ​​Leymus chinensis leaves. The results show that the correction coefficient of the leaf area calculated by the long-width method is k = 0.655 5 , which is in good agreement with the measured results. The relative error of the measurement is less than 10%, which can meet the requirements. The error is small and convenient to use. It is a method with popularization and application value. It is especially suitable for a large amount of leaf area measurement work in the field. L. chinensis generally grows in a relatively dry environment with relatively low air humidity. L. chinensis leaves wilting within 10 minutes after being isolated, which affects the accurate measurement of L. chinensis leaf area. For this reason, it is necessary to measure the results of the newly harvested leaves immediately to be as accurate as possible; if it is not possible to immediately measure, it is necessary to moisten the leaves and maintain the firmness of the leaves of the leaves, which is conducive to later measurement. The correction coefficient of leaf area is closely related to genetic factors. The leaf correction coefficient measured by the long-width method is dominated by heredity. It is not affected by the environment. The correction coefficient of each leaf is determined by different genes, although the leaf area size Affected by fertilizer and water, the correction coefficient of leaf area is relatively stable. Therefore, the value of the correction coefficient k obtained from this study can also be used under different fertilization rates and different ecological factors such as light and moisture.